Reibergram and Oligoclonal Bands in Diagnosis of Multiple Sclerosis
Jasenko Karamehic, Marina Delic-Sarac, Djemo Subasic, Tomica Jukic, Jozo Coric, Mirsad Panjeta, Zahida Drace, Lamija Zecevic, Selma Mutevelic, Nejra Dzananovic, Nerima Grcic, Amila Kešmer
Med Arh. 2012; 66(4): 222-225

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Introduction: In this study authors have analyzed the correlation between the IgG immunoglobulins in cerebrospinal fluid and the findings of oligoclonal bands on gel. Immunoglobulin IgG in cerebrospinal fluid (CSF) can be detected in neurological diseasses (infections and inflammatory neurological diseases and in demyelinating diseases, like multiple sclerosis (MS)). Quantitative IgG in CSF can be expressed by different formulae Reiber (Reiber and Felgenhauer 1987), Tourtel-lotte (Tourtellotte 1970), Schuller (Schuller and Sagar 1983) and IgG Index (Link and Tibbling 1977). In this study we used Reibergram. Qualitative CSF IgG can be measured by electrophoresis and isoelectric focusing (IEF). We used IEF for analysig CSF and seum because of its higher sensitivity. Aims of the study: To determine the correlation of immunoglobulins IgG positivity in CSF with the finding of oligoclonal bands on the gel. Material and methods:: The retrospective study based on data processed in OJ Clinical Immunology KCUS. Patients were suspicious of multiple sclerosis according to clinical findings and magnetic resonance imaging. All CSF and serum samples were processed by nephelometry, isoelectric focusing on the gel. Statistical analysis of intrathecal synthesis was also performed according to Reibergram. Results: Analyses were performed on 76 samples of cerebrospinal fluid and serum of patients from neurological clinic, suspected of multiple sclerosis. We received following results: 42 samples tested had type 1. 25 samples tested showed type 2. 3 samples had type 3. 5 samples had type 4. 1 sample had a fifth type. When we compare these results with values obtained by intrathecal synthesis of which is determined by Reibergram we obtained the following values: 16 samples had intra-thecal synthesis of 20%–60%, 9 samples had a negative value of intrathecal synthesis of 10% or less. Discussion and Conclusion: For most patients with established MS we found intrathecal humoral response, type two, and the number and arrangement of IgG bands generally does not change during the disease, because they reflect long-term non-specific immune stimulation rather than a specific immune response that during infectious disease changes (quantitatively and qualitatively).


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