Incidence and Resistotyping Profiles of Bacillus subtilis Isolated from Azadi Teaching Hospital in Duhok City, Iraq

Najim Abdulla Yassin, Alaa Muyassar Ahmad

Mat Soc Med. 2012; 24(3): 194-197

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Background: Bacillus subtilis are opportunistic, spore forming bacteria, common soil inhabitants. A resistant spore allows bacteria to endure extreme conditions of heat and desiccation in the environments promotes their survival in many instances, even in environments like hospitals.Objectives: This paper purposes to find out the incidence of Bacillus subtilis from various sources at Azadi Teaching Hospital in Duhok city, Iraq. The susceptibility test and resistotyping (antibiotypes) profile of isolates were also studied. Methods: During a period of eight months between Januarys to April, 2011, a total of 128 samples were collected from various sources and locations at Azadi Teaching Hospital in Duhok city. A sterile cotton swabs were used to collect the samples and analyzed by plating on Blood agar, Chocolate agar and MacConkey agar followed by the identification of the isolates based on their cultural characteristics and their reactions in standard biochemical tests. All the isolates were tested for antimicrobial susceptibility by the disk diffusion technique according to the Clinical and Laboratory Standards Institute guidelines on Muller Hinton Agar. Results: Out of the 128 collected samples, 84 samples yielded bacterial growth, of them 31(24.2%) were Bacillus subtilis. Moreover, other bacterial groups were also isolated and identified. The results showed that the occurrence of Bacillus subtilis was higher than the other groups of bacteria. The susceptibility test of Bacillus subtilis isolates; the organism exhibited high susceptibility rate to gentamicin (96.7%) and ciprofloxacin (93.5%) While, cefotaxime (19.3%) and ampicillin (16.2%) demonstrated the lowest percentage of susceptibility rate. Resistotyping (antibiotypes) profiles of Bacillus subtilis isolates were determined. Out of 31 isolates, 22 of them were multiple resistant and belonged to 3 resistotype patterns; resistotype 1 was predominant among isolates. Conclusion: This study shows that there is an increased rate of incidence of Bacillus subtilis in hospital environments in study area and some of these isolates were multi-drug resistant and showed different resistotyping profiles.


1. Boer AS, Diderichsen B. On the safety of Bacillus subtilis and B. amyloliquefaciens: a review. Appl Microbiol Biotechnol. 1991; 36: 1-4.

2. Garcia-Arribas ML, Rosa MC, Mosso MA. Characterization of the strains of Bacillus isolated from orally administered solid drugs. Pharm Acta Helv. 1986; 61: 303-307.

3. Sativeer SM, Weber DJ, Rutala WA, and Thomann CA. Clinical significance of Bacillus species isolated from blood cultures. South Med J. 1989; 82(6): 705-709.

4. Martha DY, Reinhart JB, and Butler LJ. Bacillus subtilis septicemia treated with penicillin. The J of Pediatrics. 1949; 35: 237-239.

5. Gill DM. Bacterial toxins: A table of lethal amounts. Microbiol Rev. 1982; 46: 86-94.

6. Oguntoyinbo FA, Sanni AI. Determination of Toxigenic Potentials of Bacillus Strains Isolated from Okpehe, a Nigerian Fermented Condiment. World J of Micro and Biotech. 2007; 23: 65-70.

7. Østensvik ØC, Heidenreich B, O’Sullivan K, Granum PE. Cytotoxic Bacillus spp. belonging to the B. cereus and B. subtilis groups in Norwegian surface waters. J of Applied Microbiology. 2004; 96: 987-993.

8. Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. Color Atlas and Textbook of Diagnostic Microbiology. Philadelphia, Washington: JB: Lippincott; 1992.

9. Clinical and Laboratory Standard Institute. Performance standards for antimicrobial susceptibility testing. M100-S16. Wayne, PA: CLSI. 2006.

10. Hawkey PM, Lewis DA. Medical bacteriology a practical approach. Oxford, England: IPL: Eynsham; 1989: 257-258.

11. Ihde DC, Armstrong D. Clinical spectrum of infection due to Bacillus species. Amer J Med. 1973; 55: 839-845.

12. Nadia G, Talat Y, Mujahi, Sumai A. Isolation, Identification and Antibiotic resistance profile of Indigenous of bacterial isolates from urinary tract infection patients. Pakistan J of Biological Sciences. 2004; 7: 2051-2054.

13. Bienvenido D, Alora MD, Corazon D. Nosocomial Infection: Clinical Features of Two Hundred Forty Three (243) Cases in the Santo Tomas University Hospital. J Microbiol Infect Dis. 1983; 12(1): 31-39.

14. Velasco E, Sousa M, Tabak D, Bouzas LF. Bacillus subtilis infection in a patient submitted to a bone marrow transplantation. Rev Paul Med. 1992; 110: 116–117.

15. Wallet F, Crunelle V, Roussel M, Furchard A, Saunier P, Courcol RJ. Bacillus subtilis as a cause of cholangitis in polycystic kidney and liver disease. Am J Gastroenterol. 1996; 91: 1477–1478.

16. Richard V, Van der Auwera P, Snoeck R, Daneau D, Meunier F. Nosocomial bacteremia caused by Bacillus species. Eur J Clin Microbiol Infect Dis.1988; 7: 783-785.

17. Esimone CO, Oleghe PO, Ibezim EC, Okeh CO, Iroha IR. Susceptibility-resistance profile of micro-rganisms isolated from herbal medicine products sold in Nigeria. African Journal of Biotechnology. 2007; 6(24): 2766-2775.

18. Balakrishnan S, John KR, Joerge MR. Antibiotic susceptibility of Bacillus spp. Isolated from shrimp (penaeus mondon) culture ponds. Indian J of Marine Sciences. 2003; 32: 81-84.

19. Belma A, Necdet S, Yavuz B. Determination of Some Properties of Bacillus Isolated from Soil. Turk J Biol. 2002; 26: 41-48.

20. Adesida SA, Coker AO, Smith SI. Resistotyping of Campylobacter jejuni. Niger Postgrad Med J. 2003; 10(4):211-215.

21. Marco RO, Gianni P, Pier EV, Piero GC. Recurrent Septicemia in an Immunocompromised Patient Due to Probiotic Strains of Bacillus subtilis. J of Clini Micro. 1998; 4: 325-326.

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